PCR
We used gels to determine the amount of DNA within each same. In Figure 1, we can see that line 7 had the most traceable DNA base pair. Figure 1 contains the 1492/533 and 515/806 extraction methods. Compared to line 1 (the ladder), line 7 had 900 or more base pairs. This is significant because the more DNA, the more accuracy of the sequencing. Figure 2 contains the NS1/ NS2 DNA extraction. The results are similar, but contain different concentrations. The 1492/533 sample had a DNA Concentration of 176.7 ng/uL. The NS1/NS2 sample had a DNA Concentration of 199.1 ng/uL. Both samples did have a baseline correction of 340 nm. All of these facts come together to prove that our process of PCR did work in magnifying the DNA.
Transformation
Once the gel had been used, we had to transfer the DNA into vectors. These vectors are circular shaped with one smaller piece missing. The DNA fills this tiny piece. Once the DNA is injected into the plasmids, we then transfer this into Petri dishes. The Petri dishes will contain E. Coli that will tell us if the DNA was inserted properly. If the DNA was properly inserted, then there should be white colonies. If the vector closed on itself, there will be blue colonies. There was one problem. The negative background had white colonies. This wasn’t supposed to happen because DNA is negatively charged. The legation was supposed to prevent the DNA from forming. So there was possible contamination or the cells were dead.
We used gels to determine the amount of DNA within each same. In Figure 1, we can see that line 7 had the most traceable DNA base pair. Figure 1 contains the 1492/533 and 515/806 extraction methods. Compared to line 1 (the ladder), line 7 had 900 or more base pairs. This is significant because the more DNA, the more accuracy of the sequencing. Figure 2 contains the NS1/ NS2 DNA extraction. The results are similar, but contain different concentrations. The 1492/533 sample had a DNA Concentration of 176.7 ng/uL. The NS1/NS2 sample had a DNA Concentration of 199.1 ng/uL. Both samples did have a baseline correction of 340 nm. All of these facts come together to prove that our process of PCR did work in magnifying the DNA.
Transformation
Once the gel had been used, we had to transfer the DNA into vectors. These vectors are circular shaped with one smaller piece missing. The DNA fills this tiny piece. Once the DNA is injected into the plasmids, we then transfer this into Petri dishes. The Petri dishes will contain E. Coli that will tell us if the DNA was inserted properly. If the DNA was properly inserted, then there should be white colonies. If the vector closed on itself, there will be blue colonies. There was one problem. The negative background had white colonies. This wasn’t supposed to happen because DNA is negatively charged. The legation was supposed to prevent the DNA from forming. So there was possible contamination or the cells were dead.