The human microbiome can be best explained as the collection of viruses, bacteria, or other microorganisms found in the mammal body. The microbiome is essential to everyday life because microbiota make up most of our body. Our body contains 100 trillion bacteria. That is a 10 to 1 ratio when compared to our body cells. Goals of this research experiment were to find the bacteria that are present in rat DNA while learning the techniques used to extract, analyze, and use PCR and 16SrDNA sequencing to determine the DNA of the bacteria. This research could be used in several situations. This could have an impact on our environment, our health, and understanding life in general. Understanding this topic is essential for processing and using something so common to achieve potential greatness. Using multiple kits and processes we were able to identify four samples. Two of the four samples were found to be Neisseria bacilliformis and Neisseria flava. Neisseria Bacilliformis is found in the upper mucous membranes of animal and mammals. Neisseria Flava is found in the upper respiratory tract. These are both known as optimistic pathogens. These are pathogens that don’t affect you in a negative way. The other specimen found is known as Mucor Racemosus. This specimen is a fungus unlike the other two which were bacteria. This research was a success. If anything could or would be changed, the changes would occur in trying to drastically decrease the steps in extracting, cloning, and sequencing the rat DNA.